2024-03-26
【學術亮點】宿主入侵型 (Host invasion type)是 Cephaleuros 的系統發育保守特徵
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Intelligent Detection: Development of expert diagnosis system for crop cultivation and management【Department of Plant Pathology / Chih-Li Wang / Associate Professor】
智慧檢測：作物栽培管理專家診斷系統開發【植病系王智立副教授】
上架日期：2023/10/23
智慧檢測：作物栽培管理專家診斷系統開發【植病系王智立副教授】
| 論文篇名 | 英文：Host invasion type is a phylogenetically conserved characteristic of Cephaleuros 中文：宿主入侵型 (Host invasion type)是 Cephaleuros 的系統發育保守特徵 |
| 期刊名稱 | Plant Disease |
| 發表年份, 卷數, 起迄頁數 | 2023, 107(10), 3222-3229 |
| 作者 | Yu-Cheng Huang; Cheng-Yu Tsai; Chih-Li Wang (王智立)* |
| DOI | 10.1094/PDIS-10-22-2338-RE |
| 中文摘要 | Cephaleuros會在許多作物上引起藻斑病。大多數藻類物種是根據其形態特徵來定義的。最近對Cephaleuros的系統發育研究表明，形態學測定與系統發育不一致。我們的研究檢視了宿主入侵型 (host invasion types) 的系統發育一致性，這是Cephaleuros分類學中最關鍵的特徵。為了確保可以從同一分離株推斷宿主入侵類型和系統發育特徵，使用顯微解剖觀察評估宿主入侵類型，並比較來自同一藻類點和/或衍生藻類培養物的 rRNA 序列。發現宿主入侵類型是保守的分類特徵並且與 Cephaleuros 系統發育一致。結果還表明，不只一種Cephaleuros 生長在同一片葉子上，且在少數情況下，多種Cephaleuros 可能生長在同一藻點上，這表明使用不同藻點進行識別可能會導致錯誤識別。Cephaleuros 分離物依宿主入侵型分為兩個物種複合體：Cephaleuros virescens具有皮下宿主入侵類型的物種複合體（CVSC）和具有細胞間宿主入侵類型的寄生頭孢菌物種複合體（CPSC）。分子系統發育分析表明，Cephaleuros 分離株聚類為 CVSC 的 14 個進化枝和 CPSC 的 3 個進化枝。這項研究也分別在台灣發現了 16 個 CVSC 和 8 個 CPSC 的新宿主。 |
| 英文摘要 | Cephaleuros species cause algal spot diseases, also known as red rust diseases, on many plants, including fruit crops. Most algal species are defined based on their morphological characteristics. Recent phylogenetic studies of Cephaleuros pecies showed that morphological determination was not congruent with phylogeny. Our study examined the phylogenetic congruence of the host invasion types (or growth habits), the most critical characteristics in the taxonomy of Cephaleuros. To ensure that host invasion types and phylogenetic characteristics could be inferred from the same isolate, host invasion types were assessed using microanatomical observation, and rRNA sequences from the same algal spot and/or the derived algal cultures were compared. Host invasion types were found to be conserved classification traits and congruent with Cephaleuros phylogeny. The results also indicated that more than one Cephaleuros species commonly grew on the same leaf or, in a few cases, the same algal spot, suggesting that identification using different algal spots could result in misidentification. The Cephaleuros isolates were separated into two species complexes by host invasion types: the Cephaleuros virescens species complex (CVSC) with subcuticular host invasion type and the Cephaleuros parasiticus species complex (CPSC) with intercellular host invasion type. Molecular phylogenetic analysis indicated that Cephaleuros isolates clustered into 14 clades of CVSC and three clades of CPSC. This study also identified 16 and eight new hosts of CVSC and CPSC in Taiwan, respectively. |
| 發表成果與AI計畫研究主題相關性 | Cephaleuros為引起作物藻斑病之病原體，對於農業生產與作物生長有很大的影響，降低作物產量，甚至造成植株落葉與枝條枯死等問題。藉由了解該病原體之系統發育特徵與其於植物體之感染途徑，可有效控制其發生與進行田間管理。該研究與其應用可於智慧檢測上，與作物專家診斷系統之知識庫內容進行結合，以進行病害診斷，並作為制訂病害防制策略之參考。 |
